JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES
VOL 27(1-2),2012

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CONTENTS
 

1.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,01-05,2012

Characterization of Physical Factors for Optimum Lovastatin Production by  Aspergillus terreus KLVB28mu21 under Solid State Fermentation

Prabhakar M1. Lingappa K*. Vivek Babu1. Amena S. Vishalakshi N. and Mahesh D.
Department of Microbiology, Gulbarga University, Gulbarga- 585106 Karnataka, India. *E-mail- lingappak_micro@rediffmail.com 1 Associate scientist, ITC Corp R&D, Bangalore, Karnataka, India.

Abstract
Lovastatin a hypocholesterolemic agent, competitively inhibits the rate limiting enzyme of cholesterol biosynthesis 3-hydroxy-3-glutaryl glutaryl coenzyme A (HMG-CoA) reductase, which catalyzes the reduction of HMG-CoA to mevalonate during cholesterol biosynthesis. We studied lovastatin production by Aspergillus terreus KLVB28mu21 strain employing Solid State Fermentation of wheat bran. Highest yield (1110 g/gm of DW of substrate) of lovastatin was obtained at moisture content 65%, pH 5.5, temperature 300C, inoculum size 1x108 spores mL-1 and bed depth 2cm.

Key words: Characterization, physical, lovastatin, Solid State Fermentation, A. terreus

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2.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,06-10,2012

Antimicrobial susceptibility pattern of Enterococcus species Isolated from clinical samples in south India.

Aajy Kumar Oli, Rajeshwari H , 1Nagaveni S, and Kelmani Chandrakanth R*
1 Department of Biotechnology, Gulbarga University, Gulbarga- 585106, India, *Email: ckelmani@gmail.com

Abstract:
Enterococci are common cause of hospital acquired infection and have become progressively more resistant to antibiotic. Among the 13 Enterococcus species ninety percentage of Enterococcal infection are due to Enterococcus faecalis .E. faecalis is a causative agent for nosocomial infection. The present study was carried out to asses the changing trends in antibiotic susceptibility of Enterococcus species isolated from clinical samples from Gulbarga region.122 samples were collected from various hospitals and diagnostic centers in Gulbarga region. The clinical isolates were identified by Facklam and Collins conventional method. The susceptibility tests were done against vancomycin, ampicillin, oxacillin, rifamycin, ciprofloxacin, tobramycin, gentamycin, teicoplanin and streptomycin by Kirby bauer method. MIC of resistant E. faecalis isolates were determined by NCCLS method. In our study, E. faecalis strains found to be 50% multi-drug resistant and the resistance was more in case of vancomycin antibiotic. The E. faecium isolates showed high resistance to gentamycin and streptomycin among the few MDR strains. E. durans were susceptible to all the antibiotic expect streptomycin and E. gallinarium were found susceptible to all antibiotics. The MIC’s determined of the 12 strains, showed the prevalence of HLAR and HLGR among the E .faecalis isolates. The present study reveals for the first time emergence of vanocmycin resistant enterococci from this part of world and indicates the magnitude of antibiotic resistance in and around the study area.

Keywords: Enterococcus spp, Enterococcus faecalis, multidrug resistance

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3.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,11-13,2012

HEALING OF BONE DEFECTS BY AUTOGENOUS PLATELET RICH PLASMA IN PEDIATRIC PATIENTS

DR C.P.CHAUDHARY, DR. RAVIKANT, DR RAVISHANKAR M. N., DR. ANURAG YADAV*!!AND DR.GUNJAN YADAV

*! DEPT. OF PEDODONTICS, CHANDRA DENTAL COLLEGE & HOPSITAL, SAFEDABAD, BARABANKI, U.P.
!!DEPT. OF ORAL & MAXILLOFACIAL SURGERY,  !DEPT. OF PEDODONTICS,*SARDAR PATEL DENTAL COLLEGE & HOPSITAL,RAIBAREILLY ROAD, LUCKNOW

ABSTRACT
OBJECTIVES – Objective of this study is to evaluate the new bone formation in bony defects after placement of  PRP and its efficacy of regenerative potential .
METHOD – Twenty five pediatric patients were selected for the study and PRP was extracted from patients own blood and placed in defect in all patients. Post operative clinical and radiographic observation was done at 1st,4th and 6th month.
RESULTS – Faster bone healing in all patients was observed.
CONCLUSION – It can be concluded by the study that PRP is a better source of bone induction growth factors and regeneration of bone can be done at faster rate which is a favorable biological response similar to natural healing process.

KEY WORDS: Platelet rich plasma [PRP], Osseous defects, Osteoinduction, Osteoconduction, Bone regeneration, Centrifugation

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4.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,14-18,2012*

 

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5.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,19-23,2012

Management of a Young edentulous patient having  Papillon  Lefévre Syndrome – a case report

1. Prof. Dilip Kumar Nath* ,  MDS, HOD, Department of Prosthodontics, Chandra Dental College & Hospital, Safedabad, Barabanki, Uttar Pradesh, India. Pin : 225003 . E.Mail:dilip_nath2006@yahoo.co.in
2. Dr. Gautam Bagchi, MDS, Senior Lecturer, Department of Prosthodontics , Chandra Dental College & Hospital, Safedabad, Barabanki, Uttar Pradesh, India.  Pin : 225003  . E.Mail: gautambagchi79@yahoo.in
3.Madhuri Nath ( Final Year BDS Student ), Chandra Dental College & Hospital  Safedabad, Barabanki, Uttar Pradesh, India.  Pin : 225003  E.mail: madhuri.saheli@gmail.com
*Corresponding Author :
Dr. Dilip Kumar Nath
9, Ashok Nagar , G.B. Marg ,Lucknow , UP . Pin : 226018, Phone : 0522 – 2636058, È: 9838 646767      
Email : dilip_nath2006@yahoo.co.in

 

ABSTRACT

Papillon-Lefevre syndrome is a rare autosomal recessive disorder in which the frequently observed manifestations are palmoplantar keratinization and premature loss of both deciduous and permanent teeth. The palmoplantar keratoderma typically has its onset between the ages of 1 and 4 years and severe periodontitis starts at the age of 3 or 4 years. The deciduous teeth frequently fall out by about age five and most of the permanent teeth may also be lost by approximately age 17. In the  present, a young,  edentulous male of this syndrome having most  the characteristic features was treated by modified Complete Denture prosthesis considering his young age and low socioeconomic status.  

Keywords: Palmoplantar hyperkeratosis, Papillon-Lefevre syndrome, Complete denture prosthesis, Preservation of residual alveolar ridge.

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6.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,24-30,2012

VIRTUAL WATER TRADE THROUGH FOOD AND VEGETABLE CROPS IN EASTERN UTTAR PRADESH, INDIA

Ravi Prakash Singh1, H. P. Singh2 and O. P. Singh3

Department of Agricultural Economics, I. Ag. Sc., Banaras Hindu University, Varanasi, India
1Research Scholar ,  2Professor, 3Assistant Professor,

ABSTRACT
Amount of water present in cereals, milk and livestock in terms of the amount of water, one of the factors of production, used to produce it. Several researchers argued in similar lines that water scarce regions can achieve high water use efficiency at the global level by importing products that have high virtual water embedded in it and exporting products that have very low water content embedded. The present study was an attempt to estimate the virtual water trade through food and vegetable crops in eastern Uttar Pradesh, India. The study was based on the primary data and it was collected through personal interview. The result suggests that the farmers are exporting higher volume of virtual water from vegetables as compared to foodgrain. In case of Mirzapur district, total irrigation water used at farm level during the year was estimated to be 1274.04 m3. Out of this, share of virtual water was 86.83 per cent, whereas in case of Varanasi district, it was 89.94 per cent. The higher virtual water trade in Varanasi district was due to the exporting water intensive crops like paddy and sugarcane.

KEY WORDS: water, cereals, milk and livestock

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7.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,31-37,2012*

 

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8.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,38-42,2012

Tooth preparation by the indirect vision with the help of mirror

 Prof Ashish Pandey, MDS*, Prof Yogesh Kamthan, MDS**,Prof Rajesh  Jhingran***
* Department of Prosthodontics, ** Department of Operative Dentistry.*** Department of Periodontics,  Chandra Dental College & Hospital, Barabanki. UP. India ,Email: ramesh pandey99@gmail.com

ABSTRACT
In the modern time everything is changing even in Dentistry lot of technique has been changed and still lot of things has to be changed. We as dentist have lot of challenges and we to change our attitude, knowledge and skill too to survive and to prove our self competitive and qualified. One thing which very important for training our budding students is how to develop our skill in those areas which are not easily accessible like if we are working in upper 2ndmolar or 3rd  molar where we can’t work directly we have to take the help of mirror for work . This type of work requires lot of skill and command. tooth preparation and cavity preparation by the help of mirror is difficult task and require lot of training to execute good work so the goal of this study was to search whether  we can  learn and improve our skill by the help of mirror inverted movement with the device  and other  whether success can be transferred to the clinical situation.  We divided four  groups of students at different levels of dental study  were asked to perform a  exercise with indirect vision using Mirror device we also tried to know that whether this technique is helpful to execute their training skill into the clinical work. In the end of this experiment we find that mirror-inverted motor functions can be easily learned and enhanced by practice. These practice sessions will make students perfect in their preclinical studies and it will be much easy for them to execute their clinical work.

Key Words: Tooth Prepration, Indirect Vision, Mirror

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9.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,43-46,2012

Comparative study of knowledge level of dental students about various kinds’ viral hepatitis.

Prof Ashish Pandey MDS. Prof Yogesh Kamthan, Prof Rajesh Jhingran
Chandra Dental College & Hospital, Barabanki. UP. India. Email: ramesh pandey99@gmail.com

ABSTRACT
The Hepatitis B virus (HBV) is an extremely infectious yet, preventable blood borne pathogen, with an estimated 350 million chronic carriers worldwide and a prevalence of 10-18 percent in surgeons. In comparison to other viruses, such as HIV, HBV differs mainly in its higher infectivity and preventability. In the clinical setting, it has been an emerging topic heated with debate specifically, over the policies governing infected health care workers who frequently perform exposure-prone procedures. In the context of dentistry, exposure-prone procedures refer to the “major cutting or removal of any oral or perioral tissue, including tooth structures. An undergraduate medical & dental students' knowledge about hepatitis is small. The medical staff providing services must be familiar with treatment as well as with epidemiological aspects of diseases (transmission, prevention and control). Therefore, it is essential to study the level of information of this group in different fields including hepatitis.  A survey of the students' knowledge about viral hepatitis was conducted in Chandra Dental College & Hospital, Safedabad, and Barabanki. UP. India.  This is a detailed study done in cross-sectional method. All the students enrolled in different academic year were selected by stratified sampling method. Questionnaire was designed to get information about their knowledge in hepatitis.  The mean scores were 36.7 and 59 of 100 regarding knowledge about hepatitis and prevention of the disease, respectively. Students had more information about hepatitis prevention than about other aspects of the disease such as mode of transmission and symptoms. There were statistically significant differences between the knowledge of hepatitis by age, academic year. This study showed no significant association between knowledge of hepatitis and sex, job, parents' education.  This study showed that students' knowledge of hepatitis was very weak. Their knowledge with respect to the prevention of the disease was higher than other aspects. 

Key Words: Knowledge Level, Hepatitis B, Dental students

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10.JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,47-52,2012*

 

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11. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,53-56,2012


BIORESORBABLE PLATES IN PEDIATRIC FACIAL TRAUMA: A REVIEW UPTO- 2011

NIKHIL MEHROTRA, C.P. CHAUDHARY*, R.K. PANDEY***,   RAVISHANKAR**, RASHMI AGARWAL**  

1 DEPARTMENT OF ORAL SURGERY,   *DEPARTMENT OF PEDODONTICS,  **DEPARTMENT OF ORAL AND MAXILLOFACIAL SURGERY,  CHANDRA DENTAL COLLEGE AND HOSPITAL BARABANKI, INDIA, E mail ramesh pandey99@gmail.com
***DEPARTMENT OF PEDODONTICS,C.S.M. MEDICAL UNIVERSITY, U.P.,LUCKNOW.INDIA,E-mail ramesh pandey99@gmail.com

ABSTRACT
Pediatric facial injuries present a unique challenge for any operating surgeon owing to the plethora of nuances associated with their management. Furthermore varied schools of thought and ever increasing advances being made in the sciences of biomechanics and biomaterials are adding to the pre exiting dichotomy of to be or not to be, as much as they are affecting the treatment guidelines

The present review is focused on the issue of use of bio-resorbable plates in cases of pediatric facial trauma as an attempt to lessen the confusion with regards to the analogy of metallic bone plating systems , in the light of concurrent research across the globe.

This review article is based on the data base of Pubmed®, assessing the ongoing development in contemporary  Pediatric Dentistry ‘Oral and Maxillofacial Surgery along with Plastic and Reconstructive surgerydata base of Pubmed®, assessing the ongoing development in contemporary  Pediatric Dentistry Oral and Maxillofacial Surgery along with Plastic and Reconstructive surgery

Key Words: Pediatric facial injuries, Surgery, Dichotomy

 

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12. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,57-58,2012 

INCEDENCE OF GINGIVITIS, PERIODONTITIS AND DENTAL CARIES IN RURAL POPULATION OF LUCKNOW /BARABANKI REGION OF U.P.,INDIA
-SHORT COMMUNICATION

Anand Kishore , Ravi Kant, C . P. CHAUDHARY    
Department of periodontics, Chandra Dental College & Hospital Safedabad, Barabanki. (U.P.)INDIA
E mail: ramesh pandey99@gmail.com

ABSTRACT
This study was designed to evaluate the incidence of periodontal disease and dental caries being the two most common disease entities in the rural population where the dental awareness and services are least available. Various factors associated with gingivitis and Periondontal disease have been used in the development of Indices measurement criteria like pocket formation, gingival inflammation, gingival bleeding and amount of distribution of plaque and calculus were taken into consideration.

The above study was conducted through organized dental camps mainly to evaluate the status of oral health and awareness of oral hygiene among rural population in the district  Lucknow / Barabanki region of Uttar Pradesh, India.

Key Words: Periodontal disease, Caries, Oral hygiene, Lucknow ,Barabanki

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13. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,59-61,2012

A COMPARATIVE  EVALUATION OF COMPRESSIVE STRENGTH, TENSILE STRENGTH AND BOND STRENGTH OF VARIOUS DENTAL MATERIALS.

Devendra Mandhyan, Nikhil Mehrotra AND Ravikant
Department of Prosthodontics, Department of Oral and Maxillofacial Surgery, Department of Pedodontics, Chandra Dental College and Hospital, Safedabad Barabanki, U.P. 225003.
E-Mail-: Mail@Chandradentalcollege.com                  

ABSTRACT
Five different dental cements (Zinc-oxide, eugenol, Zinc Phosphate, polycarboxylate, Glass ionomer & silicophosphate) were selected for their compressive strength, tensile strength & bond strength measurements. The significantly positive correlation was observed between tensile & bond strength of all the dental cement samples. The compressive & bond strength of the cements (excluding Zinc Oxide eugenol) showed no correlation.
.
Key Words: Zinc-oxide, eugenol, Zinc Phosphate, polycarboxylate, Glass ionomer ,silicophosphate, compressive strength, tensile strength and  bond strength

 

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14. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,62-64,2012

REHABILITATION OF A PATIENT WITH PALATAL PERFORATION  DUE TO PROLONGED USE OF DENTURE WITH SUCTION DISK: A CASE REPORT

Gautam Bagchi, Devendra Mandhyan and Dilip Kumar Nath ,
Department of Prosthodontics ,Chandra Dental College & Hospital,Safedabad , Barabanki , , Pin : 225003  Uttar Pradesh. INDIA E.Mail: gautambagchi79@yahoo.in E.Mail:dilip_nath2006@yahoo.co.in

ABSTRACT
Even though it is well known about the harmful effects of suction disk use in complete dentures, they are still popular in rural and semi-urban areas of India as a cheap means to achieve retention in maxillary dentures.
In this case report we present a patient with oro-nasal opening and epithelial indentation due to prolonged use of  a denture with suction disk  for over 10 years and his rehabilitation using a combined surgical and prosthodontic approach.

KEY WORDS – Suction disk, palatal perforation, surgical-prosthodontic approach, healing plate.

i

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15. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,65-68,2012

Incidence of bacterial meningitis with special reference to latex agglutination test

Abhijeet Awari  and Sunita Nighute^
Department of Microbiology, ^Department of Physiology,    Kesar SAL Medical College & Hospital Ahmedabad,. Email: abhijit.awari@yahoo.com

ABSTRACT
One hundred and fifty patients with a clinical diagnosis of pyogenic meningitis were studied. Incidence of the disease was more common in low socioeconomic group. The present study was carried out to determine the prevalence of pyogenic meningitis in our hospital and to find out the sensitivity of gram stain,   CRP and latex agglutination tests for diagnosis of pyogenic meningitis from CSF sample. Out of 150 samples studied 50 were diagnosed as pyogenic meningitis Haemophilus influenzae was the commonest organism 12 (24%) followed by the staphylococcus aureus 8 (16%) , streptococcus pneumoniae 6 (12%)  acinetobacter species 5 (10%)  coagulase negative staphylococcus 2(4%) Ecoli 3(6%)  klebsiella species 3(6%), Pseudomonas species 2(4%), Group B streptococci 1(2%), Enterococci 1(2%). The sensitivity of Gram stain and latex agglutination test was 98% and 92% respectively. Sensitivity of  CRP was 70%. As few cases in our study were treated earlier the culture positivity was 72%. Hence Gram stain  and latex agglutination test were found to be most reliable tests for diagnosis of pyogenic meningitis.

KEY WORDS: Pyogenic meningitis, Latex agglutination CRP

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16. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,69-74,2012

Role of Intuitional Constraints in  Agricultural Diversification in Mirzapur District of Uttar Pradesh

Ram Tirath, AND P.S. Badal
Department of Agricultural Economics, Institute of agricultural Science, BHU, Varanasi – 221005, INDIA
E mail:

ABSTRACT
Institutions   play an important role in so supporting agricultural production. Institutions are defined as the formal and informal rules of behavior that govern economic transaction. Formal rules refer to law and regulation enforced by government and informal institution refers to rules of behavior enforced by peers. Institutional support includes credit to access, market facilittuies , cooperatives and other input providing  facilities.  Further, the system provides medium / short term credit for capital formation in agriculture in the form of assets like wells, pump sets.  A list of all the 12 blocks of Mirzapur district was prepared and namely two blocks Pahari and Majhawa from the district were selected randomly because of one block has cropping intensity lower and other block cropping intensity higher than the average cropping intensity.  Average cropping intensity is 144.73 of all blocks .A list of all villages of selected blocks was obtained and from this list two villages namely Ramapur , Sarawa from Majhwa block and Sindhaura ,Saraiya from Pahri block were  randomly selected for the present investigation .. Thus a total of 60 farmers selected from each block. This way a total of 120, farmers of two blocks were selected for the study. Secondary data were collected from the district, block and Tehsil head quarters, Government Publications, Department’s web site.  The study pertains to agriculture year’s 2009 beginning from July1, 2009 to June 30, 2010

The socio-economic and institutional constraints were categorized into eight groups for the purpose of explanation. These groups were - institutional constraints, transport related constraints, marketing constraints, economic constraints, social constraints, technical constraints, managerial constraints and finally, financial constraints. Empirical estimated based on farmers’ survey.

An examination of role of different factor in diversification was performed using multiple linear regressions. The adjusted R2 was found to be 67 per cent meaning thereby that the selected variables were able to explain 67 per cent variation in the Dependent variable i.e. Herfindahl Index. It can be seen from the results that holding size, credit access and distance to the market were found to be significant though with varying signs. Opposite to the a priori hypothesis, bigger holding size was found to be negatively influencing diversification. This could be due to the fact that small farmers attempted to earn more through diversification with limited size of cultivable land. On the contrary, larger farmers had no such pressing compulsion. Since diversification is not only capital intensive, it also labour intensive also. Consequently, access to institutional credit was found to positively affecting diversification. Similarly, higher family was found to be positively affecting diversification not only due to labour availability but also due to economic needs.  It can thus be concluded that improvement in sources of institutional credit and improving infrastructure in form of all season pucca roads in rural areas are key to increased diversification.

KEY WORDS

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17. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,75-79,2012

Physico-chemical and chromatographic (HPTLC) Standardization of Holoptelea integrefolia (stem bark)

Danish Iqbal1*, Raju K. Pawar2, Rajeev Kr. Sharma3
1Department of Biotechnology, Integral University, Lucknow-226026, 2-3Pharmacopoeial Laboratory for Indian Medicine, Department of AYUSH, Kamla Nehru Nagar, Ghaziabad-201001, (U.P.).
E mail: danishik786@gmail.com

 ABSTRACT
Background: Holoptelea integrifolia has several medicinal properties but adulteration, inappropriate formulation, or lack of understanding of plant and drug interactions have led to adverse reactions that are sometimes life threatening or lethal. The significant popularity of HPTLC in the analytical testing and standardization of pharmaceutical, bulk drugs and herbal drugs lends its fame to the attributes.
Objective: The present communication attempts to investigate physico-chemical analysis and chromatographic (HPTLC) profiles of Holoptelea integrefolia (stem bark) (Family: Ulmaceae). Material and Method: The physico-chemical properties of aqueous extract values have been analyzed. HPTLC of Hexane extract of Holoptelea integrefolia stem bark were carried out in two lanes: - T1 – Hexane extract of Local Market Sample Ghaziabad, T2 – Hexane extract of Museum Sample of PLIM Ghaziabad, to lay down the fingerprint profile of drug by using Linomat V applicator of Camag HPTLC instrument. TLC of the drug on Silica Gel60 GF 254 pre coated plate using Toluene: Ethyl acetate (9:1) V/V as mobile phase.
Results:  Under UV Light 254 nm four spots appeared of T1 sample and seven spots appeared of T2 sample and under UV Light 366 nm three spots appeared of T1 sample and six spots appeared of T2 sample. After derivatization of TLC plate with Anisaldehyde-Sulphuric acid reagent followed by heating at 110 0C for 10 minutes; seven spots appeared. Conclusion: The study revealed specific identities for Holoptelea integrefolia (stem bark), Rf values and  colour of bands under UV light 254nm and 366nm which may play a key role in identification of plant (stem bark).

Key words; Physico-chemical, HPTLC fingerprint, Holoptelea integrefolia (stem bark), Hexane extract.

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18. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,80-92,2012

Molecular Characterization of  Cellulose Degrading Bacteria on the basis of 16S rRNA

Kaushlesh K. Yadav1* Mohd Shariq2, Sarad K. Maurya3 , Md.Nasruddin Alam4, Khurshid Ahmad2
1Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow.India
2Department of Biotechnology, Integral university, Dasauli, Kursi road, Lucknow, India
3Department of Life Science, Guru Nanak dev University, Amritsar,India
4Department of Medical Microbiology,Faculty of Medicine,National Institute of Medical Sciences University,Jaipure   Rajasthan, India.  *Corresponding author : kaushleshdv9@gmail.com
                                                                

ABSTRACT
Culturable  mesophilic (37°C) and thermophilic (60°C) cellulose degrading bacterial flora from weathered wood-like sample collected from the moist place of institute campus. Were isolated.  Cellulase activities were checked on plate by Congo red staining method. Molecular characterization  were determined by phylogenetic analysis of the 16S rRNA sequence by various bioinformatics tools like BLAST, ClustalW, Drawtree etc. The results showed 93% resemblance with Enterobacter luduwigi, Leclercia adecorboxylata,  Enterobacter cloacae and Endophytic bacterium. This cellulose is not consumed directly by the organism, but cellulose degrading bacteria has ability to degrade this cellulose molecule into its monomer called glucose.

KEY WORDS: Molecular characterization . bioinformatics tool, cellulose degrading bacteria

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19. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,93-98,2012

PRODUCTION OF L-ASPARAGINASE BY STRAIN IMPROVEMENT AND WHOLE-CELL IMMOBILIZATION OF STREPTOMYCES GULBARGENSIS

Amena S1, Lingappa K*, Prabhakar M and Vishalakshi N
*Department of Microbiology, Gulbarga University, Gulbarga-585106 Karnataka, India.
1Department of Biotechnology, Vishweshwariah College of Applied Sciences, Gulbarga-585106 Karnataka, India *Corressponding Author E-mail: lingappak_micro@rediffmail.com

ABSTRACT
In the present study, the strain Streptomyces gulbargensis was subjected to mutagenesis by physical and chemical agents like UV rays, Ethyl methanesulphonate and Ethidium Bromide in order to isolate strains capable of producing higher yields of  L-asparaginase than the prototype. A total of thirty two mutants were obtained, however among all the mutants, S. gulbargensis mu24 was found to be the most potent. The maximum L-asparaginase activities recorded with S. gulbargensis and its mutant were 30 IU and 44.7 IU respectively at 120 h of fermentation using groundnut cake extract as medium. Hence, L-asparaginase production was enhanced by 1.49 fold by mutation. Also, attempts were made to enhance the L-asparaginase production of the prototype and the mutant strains by whole-cell immobilization in gelatin. The medium with an initial pH 8.5 was inoculated with free and immobilized cells separately and subjected to fermentation at 40oC at 200 rev/min. In an immobilized cell system, the enzyme production was enhanced by 1.10 fold compared to the conventional free-cell fermentation. The immobilized cells retained their ability to produce L-asparaginase over three cycles and the activity remained between 49.4 IU-18.3 IU (mutant strain) and 33.15 IU-12.9 IU (prototype) throughout the three cycles, with the highest activity obtained during the first cycle. Hence, the gelatin-immobilized cells of S.gulbargensis mu24 can be explored as an effective biocatalyst which can be repeatedly used for maximum production of L-asparaginase.

KEY WORDS: L-asparaginase, Streptomyces gulbargensis, Mutagens, Groundnut cake extract, Immobilization

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20. JOURNAL OF RECENT ADVANCES IN APPLIED SCIENCES(JRAAS) VOL:27,99-108,2012

SYNERGISTIC EFFECTS OF OCIMUM SANCTUM EXTRACT AND ANTIBIOTICS ON METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM CLINICAL SPECIMENS

MOHAMMED IMRAN, RUBINA LAWRENCE, *MD NASRUDDIN ALAM, **MOHD SHARIQ AND EBENEZER JEYA KUMAR
Department of Microbiology and Fermentation Technology,Jacob School of Biotechnology and Bio- Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences (Deemed University), Allahabad-211007 (U.P.), INDIA
*Department of Medical Microbiology Faculty of medicine, National Institute of Medical Sciences University,Jaipur-303121, Rajasthan
**Department of Biotechnology, Integral University Dasauli,Kursi Road, Lucknow, INDIA
*Corresponding Author: e mail : nasar.2alam@gmail.com

ABSTRACT
Methicillin Resistant Staphylococcus aureus (MRSA) is a worldwide increasing problem and
difficult to cure because MRSA are resistant against almost all the clinically available antibiotics, Synergistic effects of Ocimum sanctum extract and antibiotics on MRSA isolated clinically from swabs from pus, wound, nasal cavity, burn wound at Swaroop Rani Nehru Hospital, and Nazareth Hospital, Allahabad, were explored.

MRSA were screened for resistance against selected antibiotics such as Penicillin, Gentamicin, Cephalexin, Ciprofloxacin and Tetracycline by using disc diffusion method..Synergetic effect of
acetone extract of Ocimum sanctum and antibiotics were evaluated Zone of inhibition were
increased significantly with all antibiotics.

KEY WORDS: Methicillin Resistant Staphylococcus aureus, Ocimum sanctum, Penicillin, Gentamicin, Cephalexin, Ciprofloxacin and Tetracycline

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